p3xFLAG-THAP11-rescue
(Plasmid #37001)

• Depositing Lab: Debu Chakravarti
• Publication: Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. Epub 2012 Feb 27.

Backbone

• Vector backbone: p3xFLAG-CMV14
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 6300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: THAP11
• Species: H. sapiens (human)
• Mutation: Mutations were generated at codons 273-275 converting GAA GTG AAG to GAG GTC AAA and at codons 295-297 converting GAA CTG CGT to GAG CTC CGC
• Entrez Gene: THAP11 (a.k.a. HRIHFB2206, CTG-B43a, CTG-B45d, RONIN)
• Tag / Fusion Protein:
  • 3xFLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry