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p3xFLAG-THAP11-rescue
(Plasmid #37001)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    p3xFLAG-CMV14
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 6300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    THAP11
  • Species
    H. sapiens (human)
  • Mutation
    Mutations were generated at codons 273-275 converting GAA GTG AAG to GAG GTC AAA and at codons 295-297 converting GAA CTG CGT to GAG CTC CGC
  • Entrez Gene
    THAP11 (a.k.a. HRIHFB2206, CTG-B43a, CTG-B45d, RONIN)
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV fwd
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3xFLAG-THAP11-rescue was a gift from Debu Chakravarti (Addgene plasmid # 37001)
  • For your References section:

    A Transcriptional Regulatory Role of the THAP11-HCF-1 Complex in Colon Cancer Cell Function. Parker JB, Palchaudhuri S, Yin H, Wei J, Chakravarti D. Mol Cell Biol. 2012 May;32(9):1654-70. Epub 2012 Feb 27. 10.1128/MCB.06033-11 PubMed 22371484