Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||37164||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Modifications to backbonelacZ + KanR cassette from pKOK6 cloned into pUX2424 at SalI site.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin
Copy numberHigh Copy
Gene/Insert name~1200-bp fragment from Burkholderia xenovorans LB400 cloned into pEXT20
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer NA
- 3′ sequencing primer pRcoM1bs-R (Common Sequencing Primers)
Gene/Insert nameLacZ + KanR cassette from pKOK6
Terms and Licenses
- Not Available to Industry
This strain contains Roberts lab plasmid pUX2442 which contains ~1200bp fragment from Burkholderia xenovorans LB400 with introduced terminal HindIII and SalI sites [such that HindIII – (<-rcoM1
– intergenic region – coxM1′->) – SalI] cloned into pEXT20 with the lacZ + KanR cassette from pKOK6 cloned at the SalI site such that coxM1′::lacZ->tfd<-KanR. Suitable template for amplification of the Burkholderia xenovorans RcoM1 binding region + reporter cassette.
For strain usage see attached pdf.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:UQ4876 was a gift from Gary Roberts (Addgene plasmid # 37164)
For your References section:RcoM: a new single-component transcriptional regulator of CO metabolism in bacteria. Kerby RL, Youn H, Roberts GP. J Bacteriol. 2008 May;190(9):3336-43. Epub 2008 Mar 7. 10.1128/JB.00033-08 PubMed 18326575