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UQ4876
(Bacterial strain #37164)

No maps are available for this item.

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Bacterial Strain 37164 Bacteria in agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUX2442
  • Modifications to backbone
    lacZ + KanR cassette from pKOK6 cloned into pUX2424 at SalI site.

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    ~1200-bp fragment from Burkholderia xenovorans LB400 cloned into pEXT20
  • Species
    Burkholderia xenovorans

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    LacZ + KanR cassette from pKOK6

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Depositor Comments

This strain contains Roberts lab plasmid pUX2442 which contains ~1200bp fragment from Burkholderia xenovorans LB400 with introduced terminal HindIII and SalI sites [such that HindIII – (<-rcoM1
– intergenic region – coxM1′->) – SalI] cloned into pEXT20 with the lacZ + KanR cassette from pKOK6 cloned at the SalI site such that coxM1′::lacZ->tfd<-KanR. Suitable template for amplification of the Burkholderia xenovorans RcoM1 binding region + reporter cassette.

For strain usage see attached pdf.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UQ4876 was a gift from Gary Roberts (Addgene plasmid # 37164)
  • For your References section:

    RcoM: a new single-component transcriptional regulator of CO metabolism in bacteria. Kerby RL, Youn H, Roberts GP. J Bacteriol. 2008 May;190(9):3336-43. Epub 2008 Mar 7. 10.1128/JB.00033-08 PubMed 18326575