UQ4876
(Bacterial strain #37164)

• Depositing Lab: Gary Roberts
• Publication: Kerby et al J Bacteriol. 2008 May;190(9):3336-43. Epub 2008 Mar 7.

Backbone

• Vector backbone: pUX2442
• Modifications to backbone: lacZ + KanR cassette from pKOK6 cloned into pUX2424 at SalI site.

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: ~1200-bp fragment from Burkholderia xenovorans LB400 cloned into pEXT20
• Species: Burkholderia xenovorans

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: NA
• 3′ sequencing primer: pRcoM1bs-R

Gene/Insert 2

• Gene/Insert name: LacZ + KanR cassette from pKOK6

Resource Information

• Supplemental Documents:
  • Strains for production of RcoMBx-1 proteins and assay of in vivo activity

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This strain contains Roberts lab plasmid pUX2442 which contains ~1200bp fragment from Burkholderia xenovorans LB400 with introduced terminal HindIII and SalI sites [such that HindIII – (<-rcoM1
– intergenic region – coxM1′->) – SalI] cloned into pEXT20 with the lacZ + KanR cassette from pKOK6 cloned at the SalI site such that coxM1′::lacZ->tfd<-KanR. Suitable template for amplification of the Burkholderia xenovorans RcoM1 binding region + reporter cassette.

For strain usage see attached pdf.