UQ5875
(Bacterial strain #37167)

• Depositing Lab: Gary Roberts
• Publication: Gary Roberts unpublished plasmids (unpublished)

Backbone

• Vector backbone: pUX2996 and pUX2410

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): E. Coli UQ5853
• Growth instructions: Copy number: pUX2410 High / pUX2996 Low
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: coxM1′::lacZ reporter system AND Burkholderia xenovorans RcoM1 with C-term 6xHIS tag

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pEXT20-F1
• 3′ sequencing primer: pRcoM1bs-R

Resource Information

• Supplemental Documents:
  • Strains for production of RcoMBx-1 proteins and assay of in vivo activity

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX2996 (pUX2970 with a coxM1′::lacZ reporter system such that ...vector DNA – BamHI (unique site) – “e + f” binding region – coxM1′(with unique SalI site)::lacZ-> tfd <-KanR (with 5′-end unique XhoI site) – vector DNA <-SpR...) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).

Strain is CmR, plasmid pUX2996 is KanR and SpecR and plasmid is pUX2410 is AmpR. Can be grown in media containing Amp + Kan.

For strain usage see attached pdf. Please note that this strain has not been tested functionally by Addgene.