|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||37174||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Modifications to backbone~5-kb BamHI + XhoI fragment from pUX3009 cloned into BamHI + SalI-cut pEXT20.
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name~5-kb BamHI + XhoI fragment from pUX3009 cloned into BamHI + SalI-cut pEXT20
- Cloning method Unknown
- 5′ sequencing primer NA
- 3′ sequencing primer pRcoM1bs-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
This strain contains Roberts lab plasmid pUX3056 which contains ~5-kb BamHI + XhoI fragment from pUX3009 cloned into BamHI + SalI-cut pEXT20. Useful for mutagenesis and excision of the “d + e + f”
For strain usage details see attached pdf. Please note that Addgene has not tested this plasmid functionally.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:UQ5827 was a gift from Gary Roberts (Addgene plasmid # 37174)