Addgene - pMGAHisTev5B Plasmid Data

Plasmid 37221: pMGAHisTev5B

Gene/insert name:
eIF5B
Alt name:
FUN12
Species:
S. cerevisiae (budding yeast)
Fusion protein or tag:
His
Terminal:
N terminal on insert
Mutation:
N-term truncation (contains aa 396-1002)
Vector backbone:
pSKB3
(Search Vector Database)
Modifications to Backbone:
Vector is a derivative of pET28a(+) that has the thrombin site mutated to a Tev protease site
Cloning method:
Unknown
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T7 term
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Jon Lorsch
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Reconstitution of yeast translation initiation. Acker et al (Methods Enzymol. 2007;430:111-45. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37221" in your Materials and Methods section.