Addgene - pET15b-eIF4E Plasmid Data

Plasmid 37228: pET15b-eIF4E

Gene/insert name:
eIF4E
Alt name:
CDC33
Species:
S. cerevisiae (budding yeast)
Vector backbone:
pET15b
(Search Vector Database)
Backbone manufacturer:
EMD bioscience
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5700
Promoter:
T7
Cloning site 5':
NcoI
Site destroyed during cloning:
Unknown
Cloning site 3':
BamHI
Site destroyed during cloning:
Unknown
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T7-term
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Jon Lorsch
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: The 5'-7-methylguanosine cap on eukaryotic mRNAs serves both to stimulate canonical translation initiation and to block an alternative pathway. Mitchell et al (Mol Cell. 2010 Sep 24;39(6):950-62. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37228" in your Materials and Methods section.