pCaSpeR-4 porcupine (genomic DNA)
(Plasmid
#37376)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37376 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCaSpeR-4
- Backbone size w/o insert (bp) 7855
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameporcupine
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Alt nameporc
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SpeciesD. melanogaster (fly)
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Insert Size (bp)5500
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Entrez Genepor (a.k.a. Dmel_CG6205, 15175, CG6205, Dmel\CG6205, Porc, dmPorc, l(1)17Ac, poc, porc)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pCasper-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Perrimon Lab plasmid #123
The EcoRI-XbaI genomic DNA covering the porc locus was cloned in pCaspeR4 to generate a genomic porc rescue construct.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCaSpeR-4 porcupine (genomic DNA) was a gift from Norbert Perrimon (Addgene plasmid # 37376 ; http://n2t.net/addgene:37376 ; RRID:Addgene_37376) -
For your References section:
The segment polarity gene porcupine encodes a putative multitransmembrane protein involved in Wingless processing. Kadowaki T, Wilder E, Klingensmith J, Zachary K, Perrimon N. Genes Dev. 1996 Dec 15;10(24):3116-28. 10.1101/gad.10.24.3116 PubMed 8985181