Addgene - pHL1324 Plasmid Data

Plasmid 37638: pHL1324

Gene/Insert 1
Gene/insert name:
GFP
Alt name:
green fluorescent protein
Insert size:
717
Gene/Insert 2
Gene/insert name:
TetR
Gene/Insert 3
Gene/insert name:
CsrA
Alt name:
carbon storage regulator
Species:
E. coli
Vector backbone:
pZ with ColE origin
(Search Vector Database)
Backbone manufacturer:
Lim Lab
Vector type:
Bacterial Expression
Modifications to Backbone:
Plasmid modified from Rolf Lutz and Hermann Bujard, 1997
Cloning information for Gene/Insert 1
Promoter:
pCon
Cloning site 5':
SalI
Site destroyed during cloning:
No
Cloning site 3':
NheI
Site destroyed during cloning:
No
5' sequencing primer:
CTCATGA GCGGATACAT ATTTGAA List of Sequencing Primers
3' sequencing primer:
cgcggatcc AAGGCCATCCGTCAGGATGGCCTT
Cloning information for Gene/Insert 2
Promoter:
pConNoHind
Cloning site 5':
XmaI
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
GCTGGGATTA CACATGGCAT GGAT List of Sequencing Primers
3' sequencing primer:
ccggaattc ACTGCTCACA AGAAAAAAGG CACG
Cloning information for Gene/Insert 3
Promoter:
pConNoHind
Cloning site 5':
NotI
Site destroyed during cloning:
No
Cloning site 3':
ApaI
Site destroyed during cloning:
No
5' sequencing primer:
ggccaag CTT TAATC GTACAGGGTA GTACAAATA List of Sequencing Primers
3' sequencing primer:
AGCTGATACC GCTCGCCGCA GCCGAACG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Han Lim
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37638" in your Materials and Methods section.