Addgene - pET28b-Rv2416c_wild Plasmid Data

Plasmid 37649: pET28b-Rv2416c_wild

Gene/insert name:
Rv2416c
Alt name:
Eis
Insert size:
1224
Species:
Mycobacterium tuberculosis
Entrez Gene:
eis (Rv2416c)
Fusion protein or tag:
His
Terminal:
N terminal on backbone
Vector backbone:
pET28b
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5500
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
T7 promoter List of Sequencing Primers
3' sequencing primer:
T7 terminator
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (1)
Principal Investigator:
SeWon Suh
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mycobacterium tuberculosis Eis protein initiates suppression of host immune responses by acetylation of DUSP16/MKP-7. Kim et al (Proc Natl Acad Sci U S A. 2012 May 15;109(20):7729-34. Epub 2012 Apr 30. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37649" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

23693: pDONR223-MAP3K7