Addgene - PIGAZFN-L1 Plasmid Data

Plasmid 37797: PIGAZFN-L1

Gene/insert name:
PIGA zinc finger L1
Insert size:
264
Species:
Synthetic
Vector backbone:
pST1374
(Search Vector Database)
Backbone manufacturer:
Keith Joung lab @ MGH/HMS
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5709
Modifications to Backbone:
The backbone was digested using XbaI/BamHI to insert the zinc finger sequence.
Promoter:
CMV
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
The insert and backbone were from Dr. Keith Joung's lab in Massachusetts General Hospital/Harvard Medical School.
Sequence: View sequences (3)
Principal Investigator:
Linzhao Cheng
Terms and Licenses:
MTA

Comments:

Please also cite the original article from Joung lab describing the construction of zinc finger nuclease:
Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, et al. Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell. 2008;31:294–301.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells. Zou et al (Cell Stem Cell. 2009 Jul 2. 5(1):97-110. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 37797" in your Materials and Methods section.