Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||38061||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneMSCV-N FLAGHA
- Backbone size w/o insert (bp) 8162
- Total vector size (bp) 7010
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameE4 orf3
SpeciesHuman adenovirus 5
Insert Size (bp)348
- Promoter PKG
/ Fusion Proteins
- Flag (N terminal on backbone)
- HA (N terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer LXSN 5'
- 3′ sequencing primer MSCV-N seq. rev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byM. Vidal lab
Terms and Licenses
- Not Available to Industry
Note that the Gateway cloning reaction has left several vector-based nucleotides in frame with the 3' end of the insert, adding several amino acid residues [STFLYKVVR] fused to the C-terminus of translated protein.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MSCV-N E4orf3 was a gift from Karl Munger (Addgene plasmid # 38061 ; http://n2t.net/addgene:38061 ; RRID:Addgene_38061)
For your References section:Interpreting cancer genomes using systematic host network perturbations by tumour virus proteins. Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M. Nature. 2012 Jul 26;487(7408):491-5. 10.1038/nature11288 PubMed 22810586