pFRT/TO/HIS/FLAG/HA-PRDX1
(Plasmid #38086)

• Depositing Lab: Markus Landthaler
• Publication: Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Backbone

• Vector backbone: pFRT/TO/HIS/FLAG/HA-DEST
• Backbone size w/o insert (bp): 5321
• Total vector size (bp): 5921
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PRDX1 peroxiredoxin 1
• Alt name: PAG; PAGA; PAGB; PRX1; PRXI; MSP23; NKEFA; TDPX2
• Species: H. sapiens (human)
• Insert Size (bp): 600
• GenBank ID: NM_002574.3
• Entrez Gene: PRDX1 (a.k.a. MSP23, NKEF-A, NKEFA, PAG, PAGA, PAGB, PRX1, PRXI, TDPX2)
• Tag / Fusion Protein:
  • HIS/FLAG/HA (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV
• 3′ sequencing primer: BGH

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry