Addgene - N-cadherin in pCCL-c-MNDU3c-PGK-EGFP Plasmid Data

Plasmid 38153: N-cadherin in pCCL-c-MNDU3c-PGK-EGFP

Gene/Insert 1
Gene/insert name:
N-cadherin
Insert size:
4100
Species:
H. sapiens (human)
GenBank ID:
NM_001792
Entrez Gene:
CDH2 (CD325, CDHN, CDw325, NCAD)
Gene/Insert 2
Gene/insert name:
EGFP
Vector backbone:
pCCL-c-MNDU3c-(X2)-Pgk-EGFP
(Search Vector Database)
Backbone manufacturer:
Don Kohn dkohn@mednet.ucla.edu
Vector type:
Lentiviral
Backbone size w/o insert (bp):
7900
Cloning information for Gene/Insert 1
Promoter:
MNDU3c = LTR promoter for U3 region of myeloproliferative sarcoma retrovirus MND
Cloning site 5':
EcoRI
Site destroyed during cloning:
Yes
Cloning site 3':
EcoRI
Site destroyed during cloning:
Yes
5' sequencing primer:
MSCV_rev List of Sequencing Primers
3' sequencing primer:
EGFP-N
Cloning information for Gene/Insert 2
Promoter:
PGK
Cloning method:
Unknown
5' sequencing primer:
mPGK-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Person or lab that originally
cloned the gene/insert:
purchased clone from Origene human N-cadherin in pCMV6-XL6
Sequence: View sequences (1)
Map:
View map
Map:
View map
Principal Investigator:
Nora Heisterkamp
Terms and Licenses:
MTA

Comments:

Constructed by Bin Zhang. Vector was digested with EcoRI and the site blunted by filling in with T4 DNA polymerase. N-cadherin was isolated as a blunt-end fragment by digesting N-cadherin in pCMV6-XL with BsrBI (in front of the ATG) and with SmaI (in the vector). Has good N-cadherin expression. This vector expresses EGFP from the PGK promoter and non-tagged human N-cadherin.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Increased resistance to a farnesyltransferase inhibitor by N-cadherin expression in Bcr/Abl-P190 lymphoblastic leukemia cells. Zhang et al (Leukemia. 2007 Jun;21(6):1189-97. Epub 2007 Mar 29. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 38153" in your Materials and Methods section.