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Rac1 in pAcG2T
(Plasmid #38164)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 38164 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAcG2T
  • Backbone manufacturer
    Pharmingen
  • Backbone size w/o insert (bp) 8530
  • Total vector size (bp) 9130
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rac1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • GenBank ID
  • Entrez Gene
    RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
  • Promoter polyhedron
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer GST-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Constructed by Nora Heisterkamp. Rac1 cDNA isolated from K562 cDNA library. 5' BamHI site introduced by PCR. Constructed in 3-way ligation: 0.18 kb 5' BamHI-PvuII + 3' 0.4 kb PvuII-EcoRI into pAcG2T. Makes Rac1 in insect cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Rac1 in pAcG2T was a gift from Nora Heisterkamp (Addgene plasmid # 38164 ; http://n2t.net/addgene:38164 ; RRID:Addgene_38164)