pTH738-CENBEVYslow
(Plasmid #38220)

• Depositing Lab: Tobias von der Haar
• Publication: Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.1002/embj.201385651. Epub 2013 Dec 19.

Backbone

• Vector backbone: pCENBEVY-U
• Backbone size w/o insert (bp): 6614
• Total vector size (bp): 7122
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GCN4-based single uORF-containing 5'-UTR
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 500
• Mutation: The start codons of uORFs2, 3 and4 of the yeast Gcn4 leader sequence were mutated to non-start codons.
• Promoter: TDH3 (=GPD)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (destroyed during cloning)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: M13r
• 3′ sequencing primer: M13f

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The mutated GCN4 leder sequence originated from a plasmid kindly provided by Dr Leos Valasek (Academy of Sciences of the Czech Republic, Prague, Czech Republic). Grant CM, Miller PF, Hinnebusch AG (1994)Molecular & Cellular Proteomics 14:2616-2628.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry