Addgene - pET22b-BirA-CPDSalI Plasmid Data

Plasmid 38258: pET22b-BirA-CPDSalI

Gene/insert name:
birA
Alt name:
biotin ligase
Insert size:
980
Species:
E. coli
GenBank ID:
NC_008563.1
Entrez Gene:
birA (APECO1_2494)
Fusion protein or tag:
Vibrio cholerae MARTX toxin cysteine protease domain (CPD) (aa3440-3650)
Terminal:
C terminal on backbone
Fusion protein or tag:
6xHis
Terminal:
C terminal on backbone
Mutation:
cloned from pGEX4T1-BirA
Vector backbone:
Addgene Plasmid #38251 (pET22b-CPDSalI)
(Search Vector Database)
Backbone manufacturer:
Matthew Bogyo laboratory
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
6126
Promoter:
T7
Cloning site 5':
NdeI
Site destroyed during cloning:
No
Cloning site 3':
SalI
Site destroyed during cloning:
No
5' sequencing primer:
T7 promoter List of Sequencing Primers
3' sequencing primer:
T7 terminator
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (1)
Principal Investigator:
Matthew Bogyo
Principal Investigator:
Aimee Shen
Terms and Licenses:
MTA
EMD Millipore Limited Use Label License/Brookhaven pET Assurance Letter

Comments:

Encodes untagged biotin ligase gene (birA) from E. coli fused to Vibrio cholerae MARTX toxin cysteine protease domain (CPD) to be used to purify biotin ligase.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Simplified, enhanced protein purification using an inducible, autoprocessing enzyme tag. Shen et al (PLoS One. 2009 Dec 2;4(12):e8119. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 38258" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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