pMAL-XPG
(Plasmid #39215)

• Depositing Lab: Aziz Sancar
• Publication: Matsunaga et al J Biol Chem. 1995 Sep 1;270(35):20862-9.

Backbone

• Vector backbone: pMAL-c2
• Backbone manufacturer: New England Biolabs
• Backbone size w/o insert (bp): 6646
• Total vector size (bp): 10737
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: XPG
• Alt name: ERCC5
• Alt name: excision repair cross-complementing rodent repair deficiency, complementation group 5
• Alt name: Rad2
• Species: H. sapiens (human)
• Insert Size (bp): 4091
• Mutation: Note that XPG starts at amino acid 5
• GenBank ID: NM_000123.3
• Entrez Gene: ERCC5 (a.k.a. COFS3, ERCC5-201, ERCM2, UVDR, XPG, XPGC)
• Promoter: Ptac
• Tag / Fusion Protein:
  • MBP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: MBP_F
• 3′ sequencing primer: pBAD_rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Human XPG cDNA was provided by S. G. Clarkson (University of Geneva Medical School).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry