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human EB1 in PET28a for protein expression
(Plasmid #39297)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 39297 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    PET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6173
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Can be propagated in DH5alpha. For protein expression, grow in BL21 pLysS strain. Induce with IPTG about 3 hours and purify on Nickel agarose. For the full expression and purification protocol see Tirnauer et al MBoC 2002 EB1–Microtubule Interactions in Xenopus Egg Extracts: Role of EB1 in Microtubule Stabilization and Mechanisms of Targeting to Microtubules
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EB1
  • Alt name
    MAPRE1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    804
  • Entrez Gene
    MAPRE1 (a.k.a. EB1)
  • Promoter T7
  • Tag / Fusion Protein
    • 6HIS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 promotor primer
  • 3′ sequencing primer T7 terminator primer
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Jen-Yu (technician in Barbara Bierer's lab)
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

sequenced. See Berrueta et al 1999

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    human EB1 in PET28a for protein expression was a gift from Tim Mitchison & Jennifer Tirnauer (Addgene plasmid # 39297 ; http://n2t.net/addgene:39297 ; RRID:Addgene_39297)