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anti-Notch1_E6-pBIOCAM5
(Plasmid #39344)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 39344 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBIOCAM5-3F
  • Backbone manufacturer
    McCafferty lab unpublished
  • Total vector size (bp) 7011
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    scFv-Fc-fusion specific for Notch1 (clone B9)
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Entrez Gene
    Notch1 (a.k.a. 9930111A19Rik, Mi, Mis6, N, N1, Ta, Tan1, lin, lin-12)
  • Entrez Gene
    NOTCH1 (a.k.a. AOS5, AOVD1, TAN1, hN1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • human Fc (C terminal on backbone)
    • 6xHis (C terminal on backbone)
    • 3xFLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH_rev_primer
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Binds to both mouse mNRR1 and human hNRR1 but not NRR2.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    anti-Notch1_E6-pBIOCAM5 was a gift from John McCafferty (Addgene plasmid # 39344 ; http://n2t.net/addgene:39344 ; RRID:Addgene_39344)
  • For your References section:

    Generation of anti-Notch antibodies and their application in blocking Notch signalling in neural stem cells. Falk R, Falk A, Dyson MR, Melidoni AN, Parthiban K, Young JL, Roake W, McCafferty J. Methods. 2012 Sep;58(1):69-78. doi: 10.1016/j.ymeth.2012.07.008. Epub 2012 Jul 27. 10.1016/j.ymeth.2012.07.008 PubMed 22842086