Addgene - KHBD00486 Plasmid Data

Plasmid 39591: KHBD00486

Gene/insert name:
CG5965
Alt name:
FBgn0010328
Alt name:
woc
Alt name:
without children
Insert size:
5064
Species:
D. melanogaster (fly)
Entrez Gene:
woc (Dmel_CG5965, CG5965, Dmel\CG5965, Woc)
Vector backbone:
pDONR221
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Gateway DONR vector
Backbone size w/o insert (bp):
2536
Promoter:
none
Cloning method:
Gateway Cloning
5' sequencing primer:
M13-F20 List of Sequencing Primers
3' sequencing primer:
M13R
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Bart Deplancke
Terms and Licenses:
MTA

Comments:

Please note that the ORFs in this collection were cloned open-ended (without a stop codon).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Automated protein-DNA interaction screening of Drosophila regulatory elements Korneel et al

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 39591" in your Materials and Methods section.

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