|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40238||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 12000
Vector typeCyanobacteria cloning vector
Growth in Bacteria
Gene/Insert namepsbAI promoter, luxCDE
SpeciesSynechococcus elongatus PCC 7942
Insert Size (bp)1400
- Promoter n/a
- Cloning method Restriction Enzyme
- 5′ cloning site n/a (unknown if destroyed)
- 3′ cloning site n/a (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Lux Genes: luxCDE
Directs luciferase substrate synthesis in PCC7942.
psbAI promoter fragment from pAM1469 (NotI-BglII fragment) was cloned in pAM1504 cut with NotI-BamHI, upstream luxCDE. This plasmid directs aldehyde biosynthesis in cyanobacteria.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAM1518 was a gift from Susan Golden (Addgene plasmid # 40238 ; http://n2t.net/addgene:40238 ; RRID:Addgene_40238)
For your References section:Application of bioluminescence to the study of circadian rhythms in cyanobacteria. Andersson CR, Tsinoremas NF, Shelton J, Lebedeva NV, Yarrow J, Min H, Golden SS. Methods Enzymol. 2000;305:527-42. PubMed 10812624