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LRRK2_1867_2161_L1914N_V2048T
(Plasmid #40424)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40424 Plasmid sent as bacteria in agar stab 1 $65 *

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Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LRRK2
  • Species
    H. sapiens (human)
  • Mutation
    aa1867_2161; L1914N, V2048T
  • Entrez Gene
    LRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
  • Promoter polH
  • Tags / Fusion Proteins
    • HisTev (N terminal on insert)
    • GST-Tev (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site HindIII (destroyed during cloning)
  • 5′ sequencing primer DSP2466For:GAATTTCGTCGACAGATC
  • 3′ sequencing primer DSP2466Rev:AATAACCCGGATCAGCTT
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LRRK2_1867_2161_L1914N_V2048T was a gift from Michael J Fox Foundation MJFF (Addgene plasmid # 40424)