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pRMH120
(Plasmid #40593)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40593 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p414TEF
  • Backbone size w/o insert (bp) 5414
  • Total vector size (bp) 10860
  • Modifications to backbone
    Gal4BD-CRY2PHR inserted at TEF MCS, pADH-Gal4AD-CIBFL-tADH inserted at KpnI site
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CRY2
  • Alt name
    cryptochrome 2
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1500

Gene/Insert 2

  • Gene/Insert name
    CIB1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1000

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRMH120 was a gift from Chandra Tucker (Addgene plasmid # 40593)
  • For your References section:

    Light-mediated control of DNA transcription in yeast. Hughes RM, Bolger S, Tapadia H, Tucker CL. Methods. 2012 Aug 15. 10.1016/j.ymeth.2012.08.004 PubMed 22922268