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pCMV5-rat ERK2-L73P/S151D
(Plasmid #40819)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 40819 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV5
  • Backbone manufacturer
    David Russell, UTSW
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MAPK1
  • Alt name
    ERK2
  • Alt name
    p42
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1500
  • Mutation
    L73P, S151D
  • GenBank ID
    NM_053842
  • Entrez Gene
    Mapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
  • Promoter CMV
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Melanie Cobb, UTSW
  • Terms and Licenses
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5-rat ERK2-L73P/S151D was a gift from Natalie Ahn (Addgene plasmid # 40819 ; http://n2t.net/addgene:40819 ; RRID:Addgene_40819)
  • For your References section:

    Constitutive activation of extracellular signal-regulated kinase 2 by synergistic point mutations. Emrick MA, Hoofnagle AN, Miller AS, Ten Eyck LF, Ahn NG. J Biol Chem. 2001 Dec 7;276(49):46469-79. Epub 2001 Oct 8. 10.1074/jbc.M107708200 PubMed 11591711