psiCheck-2-3 x mir-34
(Plasmid #40831)

• Purpose: Luciferase reporter
• Depositing Lab: Phillip Zamore
• Publication: Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. Epub 2011 Nov 3.

Backbone

• Vector backbone: psiCheck-2
• Backbone manufacturer: promega
• Backbone size w/o insert (bp): 6273
• Vector type: Insect Expression, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: three sites partially complementary to miR-34
• Species: D. melanogaster (fly)
• Entrez Gene: mir-34 (a.k.a. Dmel_CR43033, 34, CR33326, CR43033, Dmel\CR43033, Dmel_CR33326, Mir-34, dme-miR-34, dme-mir-34, miR-34)
• Promoter: SV40
• Tag / Fusion Protein:
  • luciferase (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: RLucF

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The psiCheck-3xmiR-34 sequences were cloned by synthesized dsDNA

S: 5'-TCGAGGTGTTGATGCTAAGGTCACTGCCAGTGTTGATGCTAAGGTCACTGCCAGTGTTGATGCTAAGGTCACTGCCAGC

AS: 5'-GGCCGCTGGCAGTGACCTTAGCATCAACACTGGCAGTGACCTTAGCATCAACACTGGCAGTGACCTTAGCATCAACACC

the triple(3x)-repeated sequence:

5'TGGCAGTGACCTTAGCATCAACAC-3'

3'ACCGTCACTGGAATCGTAGTTGTG-5'

pairs with dme-miR-34 (uggcagugugguuagcugguugug) with "seed(red) plus 3' complementary region (blue)" fashion.