|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40882||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerTurner and Weintraub (1994)
- Backbone size (bp) 4095
Modifications to backboneFour 8bp cutter restriction enzymes (AscI, PacI, FseI, AsiSI) were introduced.
Vector typeSea urchin, zebrafish, Xenopus
- Promoter SP6
/ Fusion Protein
- mOrange (C terminal on backbone)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer CTTGATTTAGGTGACACTATAG
- 3′ sequencing primer TGTTGTTAACTTGTTTATTGCA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCS2+8CmOrange was a gift from Amro Hamdoun (Addgene plasmid # 40882 ; http://n2t.net/addgene:40882 ; RRID:Addgene_40882)
For your References section:Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR) Efflux Transporters. Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A. J Biol Chem. 2012 Nov 2. 10.1074/jbc.M112.424879 PubMed 23124201
Map uploaded by the depositor.