Addgene - pcDNA-p160MBP Plasmid Data

Plasmid 41: pcDNA-p160MBP

Gene/insert name:
p160 myb binding protein
Alt name:
Mybbp1a
Alt name:
p160MBP
Insert size:
4000
Species:
M. musculus (mouse)
GenBank ID:
BC052889
Entrez Gene:
Mybbp1a (RP23-48A2.3, AL024407, AU019902, P160, p160MBP, p67MBP)
Fusion protein or tag:
myc
Terminal:
C terminal on backbone
Vector backbone:
pcDNA3.1 myc-His A
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
EcoRV
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Tom Gonda
Sequence: View sequences (3)
Principal Investigator:
Bruce Spiegelman
Terms and Licenses:
MTA

Comments:

There is a K935R mutation in this insert. The mutation is not known to affect p160MBP function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Suppression of mitochondrial respiration through recruitment of p160 myb binding protein to PGC-1alpha: modulation by p38 MAPK. Fan et al (Genes Dev 2004 Feb 1;18(3):278-89. Epub 2004 Jan 26. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 41" in your Materials and Methods section.