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pattB-3xFlag-Loqs-PB
(Plasmid #41109)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41109 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pattB
  • Backbone size w/o insert (bp) 8000
  • Total vector size (bp) 14000
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Loquacious-PB
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    6000
  • Entrez Gene
    loqs (a.k.a. Dmel_CG6866, CG6866, CG6866), Dmel\CG6866, LOQS, Loqs, R3D1, R3D1-L, R3D1-S, TRBP, cg6866, dRax, loq, r3d1)
  • Tag / Fusion Protein
    • 3xFlag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pattB-3xFlag-Loqs-PB was a gift from Phillip Zamore (Addgene plasmid # 41109 ; http://n2t.net/addgene:41109 ; RRID:Addgene_41109)
  • For your References section:

    Dicer Partner Proteins Tune the Length of Mature miRNAs in Flies and Mammals. Fukunaga R, Han BW, Hung JH, Xu J, Weng Z, Zamore PD. Cell. 2012 Oct 9. pii: S0092-8674(12)01171-3. doi: 10.1016/j.cell.2012.09.027. 10.1016/j.cell.2012.09.027 PubMed 23063653