Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pcDNA3 Plk4(Sak) wt (Nigg HR9)
(Plasmid #41165)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41165 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1/3x myc-A
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 8400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Plk4
  • Alt name
    polo-like kinase 4
  • Alt name
    SAK
  • Alt name
    STK18
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2900
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3xMyc (N terminal on backbone)
    • PreScission Site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human PLK4 cDNA amplified from expressed sequence tag AI561146 clone IMAGE:2210962 (RZPD, Berlin, Germany).
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Polymerase chain reaction was used to amplify full-length human PLK4 from the expressed sequence tag AI561146 clone IMAGE:2210962 (RZPD, Berlin, Germany). The cDNA was then subcloned into mammalian expression vector pcDNA3.1/3x myc-A providing an N-terminal Myc tag. Construct was verified by sequencing.

Addgene NGS results found additional amino acids - ESRGPV - at the very C-terminus of the insert compared to the NCBI reference [AAX43264.1], but construct should function as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 Plk4(Sak) wt (Nigg HR9) was a gift from Erich Nigg (Addgene plasmid # 41165 ; http://n2t.net/addgene:41165 ; RRID:Addgene_41165)
  • For your References section:

    The Polo kinase Plk4 functions in centriole duplication. Habedanck R, Stierhof YD, Wilkinson CJ, Nigg EA. Nat Cell Biol. 2005 Nov;7(11):1140-6. 10.1038/ncb1320 PubMed 16244668