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Purpose(Empty Backbone)
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Depositing Lab
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Citations
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 41583 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCAGGS
- Backbone size (bp) 4790
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Modifications to backbonemCherry inserted into cloning site.
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Vector typeMammalian Expression
- Promoter CMV
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Selectable markersNone
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10/P3
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Custom
- 3′ sequencing primer Custom
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS-mCherry was a gift from Phil Sharp (Addgene plasmid # 41583 ; http://n2t.net/addgene:41583 ; RRID:Addgene_41583) -
For your References section:
In vivo structure-function analysis of human Dicer reveals directional processing of precursor miRNAs. Gurtan AM, Lu V, Bhutkar A, Sharp PA. RNA. 2012 Jun;18(6):1116-22. Epub 2012 Apr 30. 10.1261/rna.032680.112 PubMed 22546613
