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IL2 pMalLP
(Plasmid #41808)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41808 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMalLP
  • Backbone size w/o insert (bp) 6700
  • Total vector size (bp) 7000
  • Modifications to backbone
    The vector is a variant of pMalp2x, with a modified MCS and an N-terminal 3C protease cleavage site.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    IL2
  • Alt name
    Interleukin 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    429
  • Entrez Gene
    IL2 (a.k.a. IL-2, TCGF, lymphokine)
  • Promoter pTac
  • Tags / Fusion Proteins
    • Maltose Binding Protein (N terminal on backbone)
    • 3C protease site (N terminal on backbone)
    • 6x His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer Mal-E
  • 3′ sequencing primer M13-65
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

This is an e. coli periplasmic expression vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IL2 pMalLP was a gift from Chris Garcia (Addgene plasmid # 41808 ; http://n2t.net/addgene:41808 ; RRID:Addgene_41808)
  • For your References section:

    Exploiting a natural conformational switch to engineer an interleukin-2 'superkine'. Levin AM, Bates DL, Ring AM, Krieg C, Lin JT, Su L, Moraga I, Raeber ME, Bowman GR, Novick P, Pande VS, Fathman CG, Boyman O, Garcia KC. Nature. 2012 Mar 25;484(7395):529-33. doi: 10.1038/nature10975. 10.1038/nature10975 PubMed 22446627