Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pAAH1
(Plasmid #41825)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41825 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAED4
  • Backbone manufacturer
    Paul Matsudaira lab
  • Backbone size (bp) 3322
  • Modifications to backbone
    Contains the gene for the E. coli dihydrofolate reductase (DHFR) with a c-terminal histag and the hygromycin resistance cassette from pAG32Made by removing the hygromycin resistance cassette from pAG32 (Euroscarf) at the BamHI and SacI sites and ligation into pAED4-eDHFR (L. W. Miller, Y. Cai, M. P. Sheetz, V. W. Cornish, Nat. Methods 2, 255 (2005)).
  • Vector type
    Bacterial Expression, Yeast Expression ; PCR template
  • Promoter T7, TEF
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We received pAED4 from another lab, but did not sign an MTA. We received the DHFR gene from Virginia Cornish.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAH1 was a gift from Melissa Moore (Addgene plasmid # 41825 ; http://n2t.net/addgene:41825 ; RRID:Addgene_41825)
  • For your References section:

    Ordered and dynamic assembly of single spliceosomes. Hoskins AA, Friedman LJ, Gallagher SS, Crawford DJ, Anderson EG, Wombacher R, Ramirez N, Cornish VW, Gelles J, Moore MJ. Science. 2011 Mar 11;331(6022):1289-95. 10.1126/science.1198830 PubMed 21393538