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pAG306-GPD-ccdB chr I
(Plasmid #41894)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41894 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAG306GPD-ccdB
  • Backbone manufacturer
    Susan Lindquist (Addgene plasmid# 14140)
  • Backbone size (bp) 6972
  • Modifications to backbone
    Added two regions of homology to S. cerevisiae Chromosome I separated by a Not I restriction site. Plasmid can be integrated into Chr I of S. cerevisiae BY background after digestion with Not I.
  • Vector type
    Yeast Expression
  • Promoter GPD
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pAG306GPD-ccdB was obtained from Addgene
  • Terms and Licenses

Depositor Comments

This plasmid was generated by ligation of a SmaI-digested fusion PCR product that contained two ~500-base-pair regions of chromosome 1 flanking a NotI site into AatII-digested pAG306-GPD-ccdB.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG306-GPD-ccdB chr I was a gift from Dan Gottschling (Addgene plasmid # 41894 ; http://n2t.net/addgene:41894 ; RRID:Addgene_41894)
  • For your References section:

    An early age increase in vacuolar pH limits mitochondrial function and lifespan in yeast. Hughes AL, Gottschling DE. Nature. 2012 Dec 13;492(7428):261-5. doi: 10.1038/nature11654. Epub 2012 Nov 21. 10.1038/nature11654 PubMed 23172144