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pEGFP VAMP3
(Plasmid #42310)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42310 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VAMP 3
  • Alt name
    vesicle-associated membrane protein 3
  • Alt name
    CEB
  • Alt name
    Cellubrevin
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_004781.3 NP_004772.1
  • Entrez Gene
    VAMP3 (a.k.a. CEB)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EGFP-C; CMV-F
  • 3′ sequencing primer SV40-pA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Full-length cDNA of the human isoform of cellubrevin, was obtained by reverse transcription-PCR on CaCo-2 mRNA using standard procedures and the following set of oligonucleotides:

5'-ATGTCTACAGGTCCAACTGCTG-3' (hcb5')
and
5'-GCTGGTTCTTCATGAAGAGACA-3' (hcb3')

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP VAMP3 was a gift from Thierry Galli (Addgene plasmid # 42310 ; http://n2t.net/addgene:42310 ; RRID:Addgene_42310)
  • For your References section:

    A novel tetanus neurotoxin-insensitive vesicle-associated membrane protein in SNARE complexes of the apical plasma membrane of epithelial cells. Galli T, Zahraoui A, Vaidyanathan VV, Raposo G, Tian JM, Karin M, Niemann H, Louvard D. Mol Biol Cell. 1998 Jun;9(6):1437-48. 10.1091/mbc.9.6.1437 PubMed 9614185