|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||42607||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Total vector size (bp) 6258
Vector typeMammalian Expression
Growth in Bacteria
Growth Strain(s)XL1 Blue
Gene/Insert namemito soybean APEX
Insert Size (bp)870
MutationK14D, W41F, E112K
- Promoter CMV
/ Fusion Protein
- V5 tag (after mito sequence) (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMVf
- 3′ sequencing primer BHGrev (Common Sequencing Primers)
This plasmid is for expression of soybean APEX W41F in the mitochondrial matrix under the CMV promoter. In this construct, APEX has a mitochondrial localization peptide (MLATRVFSLVGKRAISTSVCVRAH) fused to its N-terminus.
This plasmid is considered an improved replacement of Addgene plasmid #40309
Also see the following reference:Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy. Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky GE, Ellisman MH, Ting AY. Nat Biotechnol. 2012 Oct 21.(PMID:
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-mito-APEX was a gift from Alice Ting (Addgene plasmid # 42607)
For your References section:Proteomic Mapping of Mitochondria in Living Cells via Spatially Restricted Enzymatic Tagging. Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Science. 2013 Jan 31. 10.1126/science.1230593 PubMed 23371551