Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pcDNA3-mito-APEX
(Plasmid #42607)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42607 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mito soybean APEX
  • Species
    soybean
  • Insert Size (bp)
    870
  • Mutation
    K14D, W41F, E112K
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 tag (after mito sequence) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMVf
  • 3′ sequencing primer BHGrev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid is for expression of soybean APEX W41F in the mitochondrial matrix under the CMV promoter. In this construct, APEX has a mitochondrial localization peptide (MLATRVFSLVGKRAISTSVCVRAH) fused to its N-terminus.

This plasmid is considered an improved replacement of Addgene plasmid #40309

Also see the following reference:Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy. Martell JD, Deerinck TJ, Sancak Y, Poulos TL, Mootha VK, Sosinsky GE, Ellisman MH, Ting AY. Nat Biotechnol. 2012 Oct 21.(PMID:
23086203)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-mito-APEX was a gift from Alice Ting (Addgene plasmid # 42607)
  • For your References section:

    Proteomic Mapping of Mitochondria in Living Cells via Spatially Restricted Enzymatic Tagging. Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY. Science. 2013 Jan 31. 10.1126/science.1230593 PubMed 23371551