MAP2-CD4
(Plasmid #43916)

• Depositing Lab: John Gearhart
• Publication: Addis et al PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub 2011 Dec 9.

Backbone

• Vector backbone: FU-MAP2-Gateway
• Backbone manufacturer: Addgene plasmid 43915
• Backbone size w/o insert (bp): 9600
• Total vector size (bp): 10900
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use recA cells, such as STBL3 (Invitrogen)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CD4
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• GenBank ID: NM_000616.4 NP_000607.1
• Entrez Gene: CD4 (a.k.a. CD4mut, IMD79, Leu-3, OKT4D, T4)
• Promoter: Human MAP2 promoter

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Human CD4 cDNA sequence cloned from plasmid pMAC24-IRESII, Miltenyi Biotec,

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The MAP2-GFP lentiviral reporter plasmid pGZ-hMAP2 was purchased from System Biosciences (SR10047PA-1). The MAP2 promoter sequence from this plasmid was then cloned along with the Gateway cassette of pEF-DEST51 into the backbone of FUdeltaGW-rtTA (Addgene plasmid 19780) following removal of the ubiquitin promoter-rtTA sequence to produce FU-MAP2-Gateway (Addgene plasmid #43915).

This destination vector was subsequently used to generate MAP2-CD4 (human CD4 sequence cloned from pMACS4-IRESII, Miltenyi Biotec, Cambridge, MA).

The V5 and His tags present in the vector backbone are not expressed with the CD4 insert.