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Addgene

pInducer10-mir-RUP-PheS Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb Extend DNA ladder and a digestion of plasmid 44011 with EcoRI-HF and XhoI

We expected the following banding patterns:

EcoRI-HF single digest: 1 band @14.5kb

XhoI single digest: 1 band @14.5kb

EcoRI-HF / XhoI double digest: 2 bands: 13kb and 1.5kb