Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pEV53D
(Plasmid #44168)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 44168 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2900
  • Total vector size (bp) 10249
  • Modifications to backbone
    Removed Neo gene from pcDNA3.1
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    May grow better in E. coli strain HB101.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Equine infectious anemia virus (EIAV) gag-pol,rev, and S2
  • Species
    Genes from Wyoming strain EIAV
  • Insert Size (bp)
    5200
  • Mutation
    A131T mutation in gag; Deletion of viral LTR sequences, deletion of viral packaging signal, deletion of first exon of tat gene, extensive deletion of env gene
  • GenBank ID
    M87581
  • Promoter CMV

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr Frederick Fuller (North Carolina State University, Raleigh, NC, USA).
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

A131T mutation in gag compared to GenBank reference sequence M87581

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEV53D was a gift from John Olsen (Addgene plasmid # 44168 ; http://n2t.net/addgene:44168 ; RRID:Addgene_44168)
  • For your References section:

    High efficiency gene transfer to airways of mice using influenza hemagglutinin pseudotyped lentiviral vectors. Patel M, Giddings AM, Sechelski J, Olsen JC. J Gene Med. 2013 Jan;15(1):51-62. doi: 10.1002/jgm.2695. 10.1002/jgm.2695 PubMed 23319179