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pLV-mitoGFP
(Plasmid #44385)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 44385 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLV
  • Backbone size w/o insert (bp) 6727
  • Total vector size (bp) 7500
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Grow and plate in LB with low salt
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mitoGFP
  • Alt name
    Cox8 targeting sequence and GFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    820
  • GenBank ID
    AK311934
  • Promoter CMV
  • Tags / Fusion Proteins
    • Cox8 targeting sequence (N terminal on insert)
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-mitoGFP was a gift from Pantelis Tsoulfas (Addgene plasmid # 44385 ; http://n2t.net/addgene:44385 ; RRID:Addgene_44385)
  • For your References section:

    Mislocalization of neuronal mitochondria reveals regulation of Wallerian degeneration and NMNAT/WLDS-mediated axon protection independent of axonal mitochondria. Kitay BM, McCormack R, Wang Y, Tsoulfas P, Zhai RG. Hum Mol Genet. 2013 Jan 24. 10.1093/hmg/ddt009 PubMed 23314018