|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||44494||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2445
Vector typeGateway expression clone
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Growth instructionsThe plasmid was transformed into EPI400 cells from Epicentre and requires induction with their "copy cutter solution" for DNA isolation.
Copy numberHigh Copy
Gene/Insert nameMinimal Mos1 [256x LacO, unc-119, NeoR, peel-1]
SpeciesD. melanogaster (fly), C. elegans (nematode); Bacterial
Insert Size (bp)12458
- Cloning method Gateway Cloning
- 5′ sequencing primer M13rev
- 3′ sequencing primer M13-fwd20 (Common Sequencing Primers)
Terms and Licenses
This plasmid may contain truncated lacO repeats. The depositing lab is re-transforming the plasmid and will deposit new verified strain.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCFJ1324 was a gift from Erik Jorgensen (Addgene plasmid # 44494)