|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||44715||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6433
Modifications to backbone1)To make pJDS vectors compatible with the Voytas lab Golden Gate kit, the overhangs generated by Esp3I cleavage sites in the pJDS-GG vectors were adjusted to GGGA/CTAT to ensure proper complementarity between intermediate vectors and the final vector. 2)Each pJDS-GG vector is modified to match the reading frame of the Golden Gate assembled intermediate arrays.
Vector typeMammalian Expression
- Promoter CMV
/ Fusion Proteins
- 3X Flag (N terminal on backbone)
- FOKI WT (C terminal on backbone)
Growth in Bacteria
Copy numberHigh Copy
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
This plasmid is modified pJDS74 vector from "Joung Lab TAL Effector Engineering Reagents" Kit. Modifications are made to the backbone to make it compatible with Voytas Golden Gate TALEN Kit and it can be used in place of pTAL3 or pTAL4. Please see the attached protocol below for modifications.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJDS74-GG-NNN was a gift from Nathan Lawson (Addgene plasmid # 44715)
For your References section:Targeted Chromosomal Deletions and Inversions in Zebrafish. Gupta A, Hall VL, Kok FO, Shin M, McNulty JC, Lawson ND, Wolfe SA. Genome Res. 2013 Mar 11. 10.1101/gr.154070.112 PubMed 23478401