|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||44810||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameFREQ-Seq Barcode: GGATTA
- Cloning method Unknown
- 5′ sequencing primer pBR322ori-F (Common Sequencing Primers)
These plasmids are used to generate bridging primers with Illumina-compatible barcodes for allele frequency sequencing (FREQ-Seq). Similar to RNA-Seq, the FREQ-Seq method can rapidly determine allele frequencies directly from mixed populations with minimal library preparation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFSBC46 was a gift from Christopher Marx (Addgene plasmid # 44810 ; http://n2t.net/addgene:44810 ; RRID:Addgene_44810)
For your References section:FREQ-Seq: a rapid, cost-effective, sequencing-based method to determine allele frequencies directly from mixed populations. Chubiz LM, Lee MC, Delaney NF, Marx CJ. PLoS One. 2012;7(10):e47959. doi: 10.1371/journal.pone.0047959. Epub 2012 Oct 31. 10.1371/journal.pone.0047959 PubMed 23118913