fj89c07 #2 in pBK-CMV
(Plasmid
#44966)
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PurposeThis is intended for use in riboprobe synthesis, not for cloning.
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44966 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBK-CMV
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAngptl4
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Alt nameFiaf
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SpeciesD. rerio (zebrafish)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note from the depositor: This plasmid is an EST containing a fragment of the zebrafish angptl4 gene. There are multiple SNPs in the gene that vary between zebrafish strains. This is intended for use in riboprobe synthesis, not for cloning.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
fj89c07 #2 in pBK-CMV was a gift from John Rawls (Addgene plasmid # 44966 ; http://n2t.net/addgene:44966 ; RRID:Addgene_44966)