|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45062||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerMaurer lab
- Backbone size w/o insert (bp) 4265
- Total vector size (bp) 5675
Modifications to backboneBackbone derived from pRSV-globin
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameCam Kinase IV
SpeciesM. musculus (mouse)
Insert Size (bp)1410
- Promoter RSV
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CGATACAATAAACGCCATTTGA (Common Sequencing Primers)
*This CaMKIV has a R128A mutation. The depositor considers this a polymorphism that is not functionally relevant.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRSV-CaMKIV was a gift from Richard Maurer (Addgene plasmid # 45062 ; http://n2t.net/addgene:45062 ; RRID:Addgene_45062)
For your References section:Differential activation of CREB by Ca2+/calmodulin-dependent protein kinases type II and type IV involves phosphorylation of a site that negatively regulates activity. Sun P, Enslen H, Myung PS, Maurer RA. Genes Dev. 1994 Nov 1;8(21):2527-39. 10.1101/gad.8.21.2527 PubMed 7958915