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pBp-FGFR3c-R248C
(Plasmid #45712)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45712 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBabe puro Gateway
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7900
  • Modifications to backbone
    Gateway cloning sequences flank region with gene insert for use with the Gateway system reagents.
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    FGFR3 IIIc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • Mutation
    R248C
  • Entrez Gene
    FGFR3 (a.k.a. ACH, CD333, CEK2, HSFGFR3EX, JTK4)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer Gateway reagents
  • 3′ sequencing primer Gateway reagents
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBp-FGFR3c-R248C was a gift from Matthew Meyerson (Addgene plasmid # 45712 ; http://n2t.net/addgene:45712 ; RRID:Addgene_45712)
  • For your References section:

    Inhibitor-sensitive FGFR2 and FGFR3 mutations in lung squamous cell carcinoma. Liao RG, Jung J, Tchaicha JH, Wilkerson MD, Sivachenko A, Beauchamp EM, Liu Q, Pugh TJ, Pedamallu CS, Hayes DN, Gray NS, Getz G, Wong KK, Haddad RI, Meyerson M, Hammerman PS. Cancer Res. 2013 Jun 20. 10.1158/0008-5472.CAN-12-3950 PubMed 23786770