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Tet-O-FUW-Olig2
(Plasmid #45844)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45844 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Tet-O-FUW
  • Total vector size (bp) 9361
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Olig2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    972
  • GenBank ID
    NM_016967.2
  • Entrez Gene
    Olig2 (a.k.a. AI604895, Bhlhb, Bhlhb1, Olg, Olg-2, Oligo2, RK1, RK17, bHLHe1, bHLHe19)
  • Promoter tet-ON

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer WPRE-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tet-O-FUW-Olig2 was a gift from Marius Wernig (Addgene plasmid # 45844 ; http://n2t.net/addgene:45844 ; RRID:Addgene_45844)
  • For your References section:

    Generation of oligodendroglial cells by direct lineage conversion. Yang N, Zuchero JB, Ahlenius H, Marro S, Ng YH, Vierbuchen T, Hawkins JS, Geissler R, Barres BA, Wernig M. Nat Biotechnol. 2013 May;31(5):434-9. doi: 10.1038/nbt.2564. Epub 2013 Apr 14. 10.1038/nbt.2564 PubMed 23584610