|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45871||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 4400
Vector typein situ
Growth in Bacteria
Gene/Insert nameGRP in situ probe
Alt namegastrin releasing peptide
SpeciesM. musculus (mouse)
Insert Size (bp)439
Mutationnt 66-504 on NM_175012
Entrez GeneGrp (a.k.a. BLP)
- Cloning method TOPO Cloning
- 5′ sequencing primer M13 Rev, Sp6
- 3′ sequencing primer T7, M13 Forward (Common Sequencing Primers)
Terms and Licenses
For in vitro transcription, use the NotI restriction digest and Sp6 promoter for sense probe generation and the HindIII restriction digest and T7 promoter for antisense probe generation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCRII-Topo Grp was a gift from Jeffrey Macklis (Addgene plasmid # 45871 ; http://n2t.net/addgene:45871 ; RRID:Addgene_45871)
Map uploaded by the depositor.