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pcDNA3-HA-Nck1
(Plasmid #45892)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 45892 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nck-1
  • Alt name
    NCK1
  • Alt name
    NCK adaptor protein 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    NCK1 (a.k.a. NCK, NCKalpha, nck-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The HAtagged Nck-1 construct subcloned into pRK5 was provided by W. Li (Norris Cancer Center, University of Southern California, Los Angeles).
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

HA-Nck1 was released from provided HA-Nck1 construct with a BamHI/EcoRI digest, subcloned into HAPCRII and then released with HindIII/EcoRI and ligated into pcDNA3. Insert contains Kozak sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA-Nck1 was a gift from Louise Larose (Addgene plasmid # 45892 ; http://n2t.net/addgene:45892 ; RRID:Addgene_45892)
  • For your References section:

    Modulation of protein translation by Nck-1. Kebache S, Zuo D, Chevet E, Larose L. Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5406-11. 10.1073/pnas.082483399 PubMed 11959995