Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45892||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt nameNCK adaptor protein 1
SpeciesH. sapiens (human)
Entrez GeneNCK1 (a.k.a. NCK, NCKalpha, nck-1)
- Promoter CMV
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe HAtagged Nck-1 construct subcloned into pRK5 was provided by W. Li (Norris Cancer Center, University of Southern California, Los Angeles).
Terms and Licenses
- Not Available to Industry
HA-Nck1 was released from provided HA-Nck1 construct with a BamHI/EcoRI digest, subcloned into HAPCRII and then released with HindIII/EcoRI and ligated into pcDNA3. Insert contains Kozak sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-HA-Nck1 was a gift from Louise Larose (Addgene plasmid # 45892 ; http://n2t.net/addgene:45892 ; RRID:Addgene_45892)
For your References section:Modulation of protein translation by Nck-1. Kebache S, Zuo D, Chevet E, Larose L. Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5406-11. 10.1073/pnas.082483399 PubMed 11959995
Map uploaded by the depositor.