GST eIF2beta
(Plasmid
#45900)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 45900 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-2TK
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Backbone manufacturerGE Healthcare
- Backbone size w/o insert (bp) 4900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeIF2S2
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Alt nameeIF2beta
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Alt nameeukaryotic translation initiation factor 2, subunit 2 (beta)
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SpeciesM. musculus (mouse)
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Entrez GeneEif2s2 (a.k.a. RP23-480H10.3, 2810026E11Rik, 38kDa, AA408636, AA571381, AA986487, AW822225, D2Ertd303e, EIF2, EIF2B)
- Promoter tac
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST eIF2beta was a gift from Louise Larose (Addgene plasmid # 45900 ; http://n2t.net/addgene:45900 ; RRID:Addgene_45900) -
For your References section:
Modulation of protein translation by Nck-1. Kebache S, Zuo D, Chevet E, Larose L. Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5406-11. 10.1073/pnas.082483399 PubMed 11959995
Map uploaded by the depositor.
