Purpose(Empty Backbone) empty backbone; high expression in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45996||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 3243
Vector typeMammalian Expression
- Promoter chicken beta-actin promoter + CMV enhancer
Growth in Bacteria
Copy numberHigh Copy
pCAG-MCSn1 was derived from pDRIVE-CAG (Invivogen) by removing the LacZ cassette and inserting the multiple cloning site (MCS) from pEGFP-N1 (Clontech).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-MCSn1 was a gift from Philipp Mergenthaler (Addgene plasmid # 45996)
For your References section:Mitochondrial hexokinase II (HKII) and phosphoprotein enriched in astrocytes (PEA15) form a molecular switch governing cellular fate depending on the metabolic state. Mergenthaler P, Kahl A, Kamitz A, van Laak V, Stohlmann K, Thomsen S, Klawitter H, Przesdzing I, Neeb L, Freyer D, Priller J, Collins TJ, Megow D, Dirnagl U, Andrews DW, Meisel A. Proc Natl Acad Sci U S A. 2012 Jan 31;109(5):1518-23. doi: 10.1073/pnas.1108225109. Epub 2012 Jan 10. 10.1073/pnas.1108225109 PubMed 22233811